Determination of the rate of base-pair substitution and insertion mutations in retrovirus replication

Author:

Dougherty J P1,Temin H M1

Affiliation:

1. McArdle Laboratory, University of Wisconsin, Madison 53706.

Abstract

We recently described a protocol for determination of retrovirus mutation rates, that is, the mutation frequency in a single cycle of retrovirus replication (J.P. Dougherty and H.M. Temin, Mol. Cell. Biol. 6:4378-4395, 1987; J.P. Dougherty and H.M. Temin, p. 18-23, in J. H. Miller and M. P. Calos, ed., Gene Transfer Vectors for Mammalian Cells, 1987). We used this protocol to determine the mutation rates for defined mutations in a replicating retrovirus by using a spleen necrosis virus-based vector. We determined that the mutation rate for a single base pair substitution during replication of this avian retrovirus is 2 x 10(-5) per base pair per replication cycle and the insertion rate is 10(-7) per base pair per replication cycle. It will be possible to use this protocol to determine mutation rates for other retroviruses.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference26 articles.

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3. High mutation rate of a spleen necrosis virus-based retrovirus vector;Dougherty J. P.;Mol. Cell. Biol.,1987

4. Dougherty J. P. and H. M. Temin. 1987. Determination of retroviral vector mutation rates and a promoterless retroviral vector p. 18-23. In J. H. Miller and M. P. Calos (ed.) Gene transfer vectors for mammalian cells. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.

5. Sequence analysis of the E2 gene of a hyperglycosylated, host restricted mutant of Sindbis virus and estimation of mutation rate from frequency of revertants;Durbin R. K.;Virology,1986

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