Comparison of PCR and Culture for Screening of Vancomycin-Resistant Enterococci: Highly Disparate Results for vanA and vanB-Reference-Cited by-同舟云学术

Comparison of PCR and Culture for Screening of Vancomycin-Resistant Enterococci: Highly Disparate Results for vanA and vanB

Published:2009-12 Issue:12 Volume:47 Page:4136-4137
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Mak Anton¹,Miller Mark A.²,Chong George³,Monczak Yury³

Affiliation:

1. Department of Microbiology, McGill University Health Centre, Montreal, Quebec, Canada

2. Department of Microbiology, Jewish General Hospital, Montreal, Quebec, Canada

3. Molecular Pathology, Department of Pathology, Jewish General Hospital, Montreal, Quebec, Canada

Abstract

ABSTRACT We compared PCR to conventional culture for the detection of vancomycin-resistant enterococci (VRE) in 30,835 rectal samples over a 3-year period. The positive and negative predictive values of vanB PCR were 1.42% and 99.9%, respectively. A positive vanB result by PCR is poorly predictive and necessitates culture for differentiation of VRE-positive and -negative individuals.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01547-09

Reference14 articles.

1. Comparison of Three PCR Primer Sets for Identification of vanB Gene Carriage in Feces and Correlation with Carriage of Vancomycin-Resistant Enterococci: Interference by vanB -Containing Anaerobic Bacilli

2. Molecular Characterization of vanB Elements in Naturally Occurring Gut Anaerobes

3. Condon, S., R. Manji, F. Zhang, and C. C. Ginochio. 2008. Evaluation of a real-time PCR assay for the presumptive identification of vancomycin-resistant enterococci in rectal specimens, abstr. D-321, p. 227. Abstr. 48th Annu. Intersci. Conf. Antimicrob. Agents Chemother. (ICAAC)-Infect. Dis. Soc. Am. (IDSA) 46th Annu. Meet. American Society for Microbiology and Infectious Diseases Society of America, Washington, DC.

4. Domingo, M. C., A. Huletsky, A. Bernal, R. Giroux, D. K. Boudreau, F. J. Picard, and M. G. Bergeron. 2005. Characterization of a Tn5382-like transposon containing the vanB2 gene cluster in a Clostridium strain isolated from human faeces. J. Antimicrob. Chemother.55:466-474.

5. Gold, H. S. 2001. Vancomycin resistant enterococci: mechanism and clinical observations. Clin. Infect. Dis.33:210-219.

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