Affiliation:
1. Department of Physiology and Biophysics, Robert Wood Johnson Medical School, University of Medicine and Dentistry, New Jersey, Piscataway 08854-5635.
Abstract
The membrane-reactive, photoactivatable probe 125I-TID [3-(trifluoromethyl)-3-(m-[125I]iodophenyl)-3H-diazirine] was found to label the M protein of vesicular stomatitis virus about 40% as much as G protein in intact virions, in agreement with labeling studies with other probes. By analyzing limited tryptic digestion and specific chemical cleavage products, the label was essentially entirely localized within the first 19, and probably within the first 5 to 10, amino acid residues at the N terminus, identifying this short amphipathic segment as the likely site of interaction of M protein with the viral bilayer.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology