Targeting of the Virulence Factor Acetohydroxyacid Synthase by Sulfonylureas Results in Inhibition of Intramacrophagic Multiplication of Brucella suis-Reference-Cited by-同舟云学术

Targeting of the Virulence Factor Acetohydroxyacid Synthase by Sulfonylureas Results in Inhibition of Intramacrophagic Multiplication of Brucella suis

Published:2005-09 Issue:9 Volume:49 Page:3922-3925
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Boigegrain Rose-Anne¹,Liautard Jean-Pierre¹,Köhler Stephan¹

Affiliation:

1. Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, Pl. E. Bataillon, 34095 Montpellier, France

Abstract

ABSTRACT The acetohydroxyacid synthase (AHAS) of Brucella suis can be effectively targeted by the sulfonylureas chlorimuron ethyl and metsulfuron methyl. Growth in minimal medium was inhibited, and multiplication in human macrophages was totally abolished with 100 μM of sulfonylureas. Metsulfuron methyl-resistant mutants showed reduced viability in macrophages and reduced AHAS activity.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.49.9.3922-3925.2005

Reference17 articles.

1. Leucine auxotrophy restricts growth of Mycobacterium bovis BCG in macrophages

2. Caron, E., J. P. Liautard, and S. Köhler. 1994. Differentiated U937 cells exhibit increased bactericidal activity upon LPS activation and discriminate between virulent and avirulent Listeria and Brucella species. J. Leukoc. Biol.56:174-181.

3. Chang, A. K., and R. G. Duggleby. 1997. Expression, purification and characterization of Arabidopsis thaliana acetohydroxyacid synthase. Biochem. J.327(Pt 1):161-169.

4. Corbel, M. J. 1997. Brucellosis: an overview. Emerg. Infect. Dis.3:213-221.

5. Delrue, R. M., P. Lestrate, A. Tibor, J. J. Letesson, and X. De Bolle. 2004. Brucella pathogenesis, genes identified from random large-scale screens. FEMS Microbiol. Lett.231:1-12.

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