Multicenter evaluation of proposed standardized procedure for antifungal susceptibility testing of filamentous fungi-Reference-Cited by-同舟云学术

Multicenter evaluation of proposed standardized procedure for antifungal susceptibility testing of filamentous fungi

Published:1997-01 Issue:1 Volume:35 Page:139-143
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Espinel-Ingroff A¹,Bartlett M¹,Bowden R¹,Chin N X¹,Cooper C¹,Fothergill A¹,McGinnis M R¹,Menezes P¹,Messer S A¹,Nelson P W¹,Odds F C¹,Pasarell L¹,Peter J¹,Pfaller M A¹,Rex J H¹,Rinaldi M G¹,Shankland G S¹,Walsh T J¹,Weitzman I¹

Affiliation:

1. Medical College of Virginia/Virginia Commonwealth University, Richmond 23298-0049, USA.

Abstract

A multicenter study was conducted to expand the generation and analysis of data that supports the proposal of a reference method for the antifungal susceptibility testing of filamentous fungi. Broth microdilution MICs of amphotericin B and itraconazole were determined in 11 centers against 30 coded duplicate pairs of Aspergillus spp., Fusarium spp., Pseudallescheria boydii, and Rhizopus arrhizus. The effect of inoculum density (approximately 10(3) and 10(4) CFU/ml), incubation time (24, 48, and 72 h), and procedure of MIC determination (conventional and colorimetric [Alamar Blue] evaluation of growth inhibition) on intra- and interlaboratory agreement was analyzed. Based on intra- (97 to 100%) and interlaboratory (94 to 95%) agreement for both drugs, the overall optimal testing conditions identified were determination of colorimetric MICs after 48 to 72 h of incubation with an inoculum density of approximately 10(4) CFU/ml. These testing conditions are proposed as guidelines for a reference broth microdilution method.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.35.1.139-143.1997

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