Overestimation of the Abundance of Sulfate-Reducing Bacteria in Human Feces by Quantitative PCR Targeting the Desulfovibrio 16S rRNA Gene-Reference-Cited by-同舟云学术

Overestimation of the Abundance of Sulfate-Reducing Bacteria in Human Feces by Quantitative PCR Targeting the Desulfovibrio 16S rRNA Gene

Published:2011-05-15 Issue:10 Volume:77 Page:3544-3546
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Christophersen C. T.¹²,Morrison M.¹³,Conlon M. A.¹²

Affiliation:

1. Preventative Health National Research Flagship, Adelaide, South Australia, Australia

2. CSIRO Food and Nutritional Sciences, Adelaide, South Australia, Australia

3. CSIRO Livestock Industries, St. Lucia, Queensland, Australia

Abstract

ABSTRACT The dominant genus of sulfate-reducing bacteria (SRB) in humans is Desulfovibrio , and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting two genes involved in SRB energy metabolism.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02851-10

Reference27 articles.

1. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

2. Real-time polymerase chain reaction quantification of specific butyrate-producing bacteria, Desulfovibrio and Enterococcus faecalis in the feces of patients with colorectal cancer;Balamurugan R.;J. Gastroenterol. Hepatol.,2008

3. Biochemistry, physiology and biotechnology of sulphate-reduring bacteria;Barton L. L.;Adv. Appl. Microbiol.,2009

4. Phylogeny of sulfate-reducing bacteria;Castro H. F.;FEMS Microbiol. Ecol.,2000

5. Antagonistic effects of sulfide and butyrate on proliferation of colonic mucosa;Christl S. U.;Dig. Dis. Sci.,1996

Cited by 34 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Fucose modifies short chain fatty acid and H2S formation through alterations of microbial cross-feeding activities;FEMS Microbiology Ecology;2023-09-19

2. Pathogenetic Effects of Bacteria of the Genus <i>Desulfovibrio</i>. Experimental Study. Part One: Iron Metabolism;Problems of Particularly Dangerous Infections;2023-07-14

3. The interplay between herbal medicines and gut microbiota in metabolic diseases;Frontiers in Pharmacology;2023-03-24

4. Dynamics of the gut-liver axis in rats with varying fibrosis severity;International Journal of Biological Sciences;2022

5. Structural and Functional Modulation of Gut Microbiota by Jiangzhi Granules during the Amelioration of Nonalcoholic Fatty Liver Disease;Oxidative Medicine and Cellular Longevity;2021-12-20