Abstract
Charcoal is an effective replacement for serum in media for the isolation and culture of Renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmonid fish. The medium, KDM-C, contains 10 g of peptone, 0.5 g of yeast extract, 1 g of L-cysteine hydrochloride, 1 g of activated charcoal, and 15 g of agar per liter and is adjusted to pH 6.8 with NaOH before autoclaving. Eight strains of R. salmoninarum grew from dilute inocula as well on KDM-C as on a standard serum-containing medium (KDM-2). The medium was effective for both primary isolations from fish and repeated transfers and has potential value for antigen preparation and physiological studies.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference17 articles.
1. Selective isolation of Renibacterium salmoninarum;Austin B.;FEMS Microbiol. Lett.,1983
2. Diversity among strains causing bacterial kidney disease in salmonid fish;Austin B.;Curr. Microbiol.,1980
3. Protein-free and lowprotein media for the cultivation of Leptospira;Bey R. F.;Infect. Immun.,1978
4. Corynebacterial kidney disease of salmonids: growth and serological studies on the causative bacterium;Bullock G. L.;Appl. Microbiol.,1974
5. Earp B. J. C. H. Ellis and E. J. Ordal. 1953. Kidney disease in young salmon. Special Report Series 1. State of Washington Department of Fisheries Olympia.
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