Detection of Listeria monocytogenes by using the polymerase chain reaction-Reference-Cited by-同舟云学术

Detection of Listeria monocytogenes by using the polymerase chain reaction

Published:1990-09 Issue:9 Volume:56 Page:2930-2932
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Bessesen M T¹,Luo Q A¹,Rotbart H A¹,Blaser M J¹,Ellison R T¹

Affiliation:

1. Medical Service, Department of Veterans Affairs Medical Center, Denver, Colorado.

Abstract

A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.56.9.2930-2932.1990

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