Affiliation:
1. Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA
Abstract
ABSTRACT
Research on
Legionella pneumophila
, the causative agent of Legionnaires' disease, has been hampered due to the lack of selectable markers for genetic manipulation. We report the construction of a mutant strain of
L. pneumophila
lacking
loxA
, a chromosomally encoded β-lactamase, that has enhanced sensitivity to ampicillin. Also described are a method for converting
Legionella
strains to ampicillin sensitivity and conditions for utilizing
bla
as a selectable marker.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
1 articles.
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