Behavior of Foodborne Pathogens Listeria monocytogenes and Staphylococcus aureus in Mixed-Species Biofilms Exposed to Biocides

Author:

Oxaran Virginie1ORCID,Dittmann Karen Kiesbye1ORCID,Lee Sarah H. I.2,Chaul Luíza Toubas3,Fernandes de Oliveira Carlos Augusto2,Corassin Carlos Humberto2,Alves Virgínia Farias3,De Martinis Elaine Cristina Pereira4,Gram Lone1ORCID

Affiliation:

1. Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark

2. FZEA/USP, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil

3. FF/UFG, Faculty of Pharmacy, Federal University of Goiás, Goiânia, Brazil

4. FCFRP/USP, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

Abstract

This study demonstrates that foodborne pathogens can be established in mixed-species biofilms and that this can protect them from biocide action. The protection is not due to specific characteristics of the pathogen, here S. aureus and L. monocytogenes , but likely caused by specific members or associations in the mixed-species biofilm. Biocide treatment and resistance are a challenge for many industries, and biocide efficacy should be tested on microorganisms growing in biofilms, preferably mixed systems, mimicking the application environment.

Funder

Sao Paulo Research Foundation

Brazilian Federal Agency for Suport and Evaluation and Graduate Education

Innovationsfonden

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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