Genetic Evidence that InhA of Mycobacterium smegmatis Is a Target for Triclosan-Reference-Cited by-同舟云学术

Genetic Evidence that InhA of Mycobacterium smegmatis Is a Target for Triclosan

Published:1999-03 Issue:3 Volume:43 Page:711-713
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

McMurry Laura M.¹,McDermott Patrick F.¹,Levy Stuart B.¹²

Affiliation:

1. Center for Adaptation Genetics and Drug Resistance, and Departments of Molecular Biology and Microbiology1 and

2. Medicine,2 Tufts University School of Medicine, Boston, Massachusetts 02111

Abstract

ABSTRACT Three Mycobacterium smegmatis mutants selected for resistance to triclosan each had a different mutation in InhA, an enoyl reductase involved in fatty acid synthesis. Two expressed some isoniazid resistance. A mutation originally selected on isoniazid also mediated triclosan resistance, as did the wild-type inhA gene on a multicopy plasmid. Replacement of the mutant chromosomal inhA genes with wild-type inhA eliminated resistance. These results suggest that M. smegmatis InhA, like its Escherichia coli homolog FabI, is a target for triclosan.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.43.3.711

Reference18 articles.

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