Designed Ankyrin Repeat Protein (DARPin) Neutralizers of TcdB from Clostridium difficile Ribotype 027-Reference-Cited by-同舟云学术

Designed Ankyrin Repeat Protein (DARPin) Neutralizers of TcdB from Clostridium difficile Ribotype 027

Published:2019-10-30 Issue:5 Volume:4 Page:
ISSN:2379-5042
Container-title:mSphere
language:en
Short-container-title:mSphere

Author:

Peng Zeyu¹,Simeon Rudo¹,Mitchell Samuel B.¹,Zhang Junjie²,Feng Hanping³,Chen Zhilei¹

Affiliation:

1. Department of Microbial Pathogenesis and Immunology, Texas A&M University Health Science Center, College Station, Texas, USA

2. Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA

3. Department of Microbial Pathogenesis, University of Maryland Dental School, Baltimore, Maryland, USA

Abstract

We report the engineering and characterization of designed ankyrin proteins as potent neutralizers of TcdB toxin secreted by a hypervirulent ribotype 027 strain of Clostridium difficile . We further show that although TcdB toxins from both ribotype 027 and VPI 10461 interact efficiently with TcdB receptors CSPG4 and Pvrl3, TcdB 027 lacks significant ability to bind the only known physiologically relevant TcdB receptor, Frizzled 1/2/7.

Funder

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSphere.00596-19

Reference57 articles.

1. The role of toxin A and toxin B in the virulence of Clostridium difficile

2. Clostridium difficile Toxin Biology

3. Burden ofClostridium difficileInfection in the United States