Automated Enzymatic Method for the Determination of Ammonia: Application to Rumen Fluid, Gut Fluid, and Plasma-Reference-Cited by-同舟云学术

Automated Enzymatic Method for the Determination of Ammonia: Application to Rumen Fluid, Gut Fluid, and Plasma

Published:1979-08 Issue:2 Volume:38 Page:212-215
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Baetz Albert L.¹,Faidley Terry D.¹,Allison Milton J.¹

Affiliation:

1. Biochemistry and Physiology Sections, National Animal Disease Center, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, Ames, Iowa 50010

Abstract

The enzymatic method for the determination of ammonia with glutamate dehydrogenase has been adapted to the AutoAnalyzer. The automated method was applicable for NH 3 measurement in sheep rumen samples, swine gut fluid samples, and bovine plasma. Results were compared with those of the Conway diffusion method and the manual enzymatic method. The automated method allows 30 samples per h to be analyzed routinely. Values were comparable with those with the manual method, but lower than those with the Conway diffusion method.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.38.2.212-215.1979

Reference5 articles.

1. Conway E. J. 1962. Ammonia. General method p. 98-100. In Microdiffusion analysis and volumetric error. Crosby Lockwood and Son Ltd. London.

2. Enzymatic determination of ammonia in blood plasma;Ishihara A.;Clin. Chim. Acta,1972

3. An enzymatic determination of ammonia in biological fluids;Mondzac A.;J. Clin. Med.,1965

4. An enzymatic assay for ammonia in waste matter;Robbins J. E.;J. Agric. Food Chem.,1977

5. An enzymatic fluorometric method for ammonia determination;Rubin M.;Clin. Chim. Acta,1967

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