Transformation of Streptococcus lactis Protoplasts by Plasmid DNA

Author:

Kondo Jeffery K.1,McKay Larry L.1

Affiliation:

1. Department of Food Science and Nutrition, University of Minnesota, St. Paul, Minnesota 55108

Abstract

Polyethylene glycol-treated protoplasts prepared from Streptococcus lactis LM3302, a lactose-negative (Lac ) derivative of S. lactis ML3, were transformed to lactose-fermenting ability by a transductionally shortened plasmid (pLM2103) coding for lactose utilization.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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