Affiliation:
1. Department of Physics
2. Department of Molecular Biology, Princeton University, Princeton, New Jersey
3. Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania
Abstract
ABSTRACT
We show that for two well-characterized regulatory circuits in
Escherichia coli
, Tn
10
tetracycline resistance and porin osmoregulation, the transcriptional outputs in individual cells are graded functions of the applied stimuli. These systems are therefore examples of naturally occurring regulatory circuits that exhibit continuous control of transcription. Surprisingly, however, we find that porin osmoregulation is open loop; i.e., the porin expression level does not feed back into the regulatory circuit. This mode of control is particularly interesting for an organism such as
E. coli
, which proliferates in diverse environments, and raises important questions regarding the biologically relevant inputs and outputs for this system.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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