Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine-Reference-Cited by-同舟云学术

Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine

Published:1998-02 Issue:2 Volume:36 Page:569-572
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Edelstein Reva E.¹,Nickerson Deborah A.²,Tobe Vincent O.²,Manns-Arcuino Laura A.¹,Frenkel Lisa M.¹

Affiliation:

1. Division of Infectious Diseases, Department of Pediatrics,1 and

2. Department of Molecular Biotechnology,2 University of Washington, Seattle, Washington

Abstract

ABSTRACT This report describes the detection of mutations in the pol gene of human immunodeficiency virus type 1 associated with resistance to zidovudine, didanosine, and lamivudine by genotyping by an oligonucleotide ligation assay specific codons in the pol gene amplified by PCR. Our studies demonstrate the sensitivity, simplicity, and specificity of this genotyping system.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.2.569-572.1998

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