Escherichia coli peptide methionine sulfoxide reductase gene: regulation of expression and role in protecting against oxidative damage

Author:

Moskovitz J1,Rahman M A1,Strassman J1,Yancey S O1,Kushner S R1,Brot N1,Weissbach H1

Affiliation:

1. Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.

Abstract

The Escherichia coli peptide methionine sulfoxide reductase gene (msrA) encodes a single-subunit polypeptide of 212 amino acid residues (M. A. Rahman, H. Nelson, H. Weissbach, and N. Brot, J. Biol. Chem. 267:15549-15551, 1992). RNA blot analysis showed that the gene is transcribed into an mRNA of about 850 nucleotides. The promoter region was characterized, and the transcription initiation site was identified by primer extension. The synthesis of the MsrA protein increased about threefold in a growth-phase-dependent fashion. In an attempt to define the in vivo role of msrA, a chromosomal disruption was constructed. This mutant was more sensitive to oxidative stress, suggesting that oxidation of methionine in proteins plays an important role in oxidative damage.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference30 articles.

1. Enzymatic reduction of oxidized ~-1-proteinase inhibitor restores biological activity;Abrams W. R.;Proc. Natl. Acad. Sci. USA,1981

2. Induction of growth-phase-dependent promoter triggers transcription of bolA an Escherichia coli morphogene;Aldea M.;EMBO J.,1989

3. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl (ed.). 1989. Current protocols in molecular biology. Greene Publishing Associates and Wiley-Interscience New York.

4. Brot N. and H. Weissbach. 1988. Methionine sulfoxide: chemistry and biochemistry p. 851-872. In S. Patai and Z. Rappoport (ed.) The chemistry of sulphones and sulphoxides. John Wiley & Sons Inc. New York.

5. Enzymatic reduction of protein-bound methionine sulfoxide;Brot N.;Proc. Natl. Acad. Sci. USA,1981

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3