Activation of the Pseudomonas aeruginosa Type III Secretion System Requires an Intact Pyruvate Dehydrogenase aceAB Operon-Reference-Cited by-同舟云学术

Activation of the Pseudomonas aeruginosa Type III Secretion System Requires an Intact Pyruvate Dehydrogenase aceAB Operon

Published:2002-07 Issue:7 Volume:70 Page:3973-3977
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Dacheux Denis¹²,Epaulard Olivier¹³,de Groot Arjan¹,Guery Benoit⁴,Leberre Rozen⁴,Attree Ina¹,Polack Benoit³,Toussaint Bertrand¹⁵

Affiliation:

1. Département de Biologie Moléculaire et Structurale, BBSI, UMR 5092 CNRS, CEA-Grenoble

2. CHU-Grenoble, Grenoble, Université de Bordeaux, Paris, and

3. GREPI EA 2938 MENRT

4. Réanimation Médicale et Maladies Infectieuses, CH Dron, Tourcoing, France

5. DBPC/Enzymologie,

Abstract

ABSTRACT Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn 5 Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred. Expression in trans of ExsA restored TTSS function and cytotoxicity.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.70.7.3973-3977.2002

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