Affiliation:
1. Armstrong Laboratory, Tyndall Air Force Base, Florida 32403-6001.
Abstract
The degradation of p-nitrophenol (PNP) by Moraxella and Pseudomonas spp. involves an initial monooxygenase-catalyzed removal of the nitro group. The resultant hydroquinone is subject to ring fission catalyzed by a dioxygenase enzyme. We have isolated a strain of an Arthrobacter sp., JS443, capable of degrading PNP with stoichiometric release of nitrite. During induction of the enzymes required for growth on PNP, 1,2,4-benzenetriol was identified as an intermediate by gas chromatography-mass spectroscopy (GC-MS) and radiotracer studies. 1,2,4-Benzenetriol was converted to maleylacetic acid, which was further degraded by the beta-ketoadipate pathway. Conversion of PNP to 1,2,4-benzenetriol is catalyzed by a monooxygenase system in strain JS443 through the formation of 4-nitrocatechol, 4-nitroresorcinol, or both. Our results clearly indicate the existence of an alternative pathway for the biodegradation of PNP.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference15 articles.
1. Carlton B. C. and B. J. Brown. 1981. Gene mutation p. 222-242. In P. Gerhardt R. G. E. Murray R. N. Costilow E. W. Nester W. A. Wood N. R. Krieg and G. B. Phillips (ed.) Manual of methods for general bacteriology. American Society for Microbiology Washington D. C.
2. The metabolism of thymol by a Pseudomonas;Chamberlain E. M.;Biochem. J.,1968
3. Metabolism of resorcinylic compounds by bacteria: orcinol pathway in Pseudomonas putida;Chapman P. J.;J. Bacteriol.,1976
4. Metabolism of resorcinylic compounds by bacteria: alternative pathways for resorcinol catabolism in Pseudomonas putida;Chapman P. J.;J. Bacteriol.,1976
5. Degradation and induction specificity in Actinomycetes that degradep-nitrophenol;Hanne L. F.;Appl. Environ. Microbiol.,1993
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