Affiliation:
1. Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
Abstract
Tn10 insertion in the galS (ultrainduction factor) gene of Escherichia coli allows the gal operon to be constitutively expressed at a very high level, equal to that seen in a delta galR strain in the presence of an inducer. The insertion has been mapped by criss-cross Hfr matings and by marker rescue into Kohara phages at 46 min on the E. coli chromosome.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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