Use of chemotaxonomy as an aid to differentiate among Capnocytophaga species, CDC group DF-3, and aerotolerant strains of Leptotrichia buccalis

Author:

Bernard K1,Cooper C1,Tessier S1,Ewan E P1

Affiliation:

1. National Laboratory for Bacteriology, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.

Abstract

Four strains of fastidious gram-negative rods, thought to be Capnocytophaga species (formerly CDC group DF-1 or Bacteroides ochraceus) or CDC group DF-3 on the basis of conventional phenotypic criteria, were also analyzed for cellular fatty acid (CFA) composition. It was found that the CFA compositions of these strains were qualitatively incorrect for those taxa. Subsequently, it was determined that all four bacteria were in fact aerotolerant strains of Leptotrichia buccalis, based on biochemical reactions, CFA composition, and lactic acid as the major end product of glucose fermentation. It is recommended that, in addition to conventional cultural and biochemical criteria, all strains of Capnocytophaga or CDC group DF-3 should also be tested for metabolic end products of fermentation and CFA composition as essential adjuncts for identification.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference10 articles.

1. Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods;Bernard K. A.;J. Clin. Microbiol.,1991

2. Clark W. A. D. G. Hollis R. E. Weaver and P. Riley. 1984. Identification of unusual pathogenic gram-negative aerobic and facultatively anaerobic bacteria. Centers for Disease Control Atlanta.

3. Cooper C. Unpublished data.

4. Cellular fatty acids of Capnocytophaga species;Dees S. B.;J. Clin. Microbiol.,1982

5. Leptotrichia buccalis isolated from pertioneal fluid of two immunocompromised patients;Grollier G.;Clin. Microbiol. Newsl.,1990

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