Purification and Characterization of an NAD-Malic Enzyme from Bradyrhizobium japonicum A1017-Reference-Cited by-同舟云学术

Purification and Characterization of an NAD-Malic Enzyme from Bradyrhizobium japonicum A1017

Published:1998-10 Issue:10 Volume:64 Page:4073-4075
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Chen Fan¹,Okabe Youichi¹,Osano Kaoru¹,Tajima Shigeyuki¹

Affiliation:

1. Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Ikenobe 2393, Kita-gun, Kagawa 761-07, Japan

Abstract

ABSTRACT An NAD-malic enzyme was purified to homogeneity from Bradyrhizobium japonicum A1017, and its molecular characteristics were surveyed. The enzyme exhibited native and subunit molecular masses of 388 and 85 kDa, respectively, suggesting that it exists as a homotetramer, and was activated by metabolic intermediates in glycolysis. The role of the enzyme in bacteroids’ carbon metabolism is discussed.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.64.10.4073-4075.1998

Reference21 articles.

1. Basic local alignment search tool.;Altschul S. F.;J. Mol. Biol.,1990

2. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding.;Bradford M. M.;Anal. Biochem.,1976

3. Purification and characterization of the NADP-malic enzyme from Bradyrhizobium japonicum A1017.;Chen F.;Biosci. Biotech. Biochem.,1997

4. Malic enzyme activity in bacteroids from soybean nodules.;Copeland L.;J. Gen. Microbiol.,1989

5. Day D. A. Quinnell R. G. Bergersen F. J. An hypothesis on the role of malic enzyme in symbiotic nitrogen fixation in soybean nodules Symbiotic nitrogen fixation. Graham P. H. Sadowsky M. J. Vance C. P. 1994 159 164 Kluwer Academic Publishers Dordrecht The Netherlands

Cited by 20 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The C4 cycle and beyond: diverse metabolic adaptations accompany dual-cell photosynthetic functions in Setaria;Journal of Experimental Botany;2021-08-17

2. The PEP-pyruvate-oxaloacetate node: variation at the heart of metabolism;FEMS Microbiology Reviews;2020-12-08

3. Role of the malic enzyme in metabolism of the halotolerant methanotroph Methylotuvimicrobium alcaliphilum 20Z;PLOS ONE;2019-11-18

4. High resolution two-dimensional electrophoresis of native proteins;ELECTROPHORESIS;2014-04-16

5. Breeding of a Cyclic Imide-Assimilating Bacterium,Pseudomonas putidas52, for High Efficiency Production of Pyruvate;Bioscience, Biotechnology, and Biochemistry;2013-08-23