Detection and Quantification of Dehalogenimonas and “ Dehalococcoides ” Populations via PCR-Based Protocols Targeting 16S rRNA Genes-Reference-Cited by-同舟云学术

Detection and Quantification of Dehalogenimonas and “ Dehalococcoides ” Populations via PCR-Based Protocols Targeting 16S rRNA Genes

Published:2009-12 Issue:23 Volume:75 Page:7560-7564
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Yan Jun¹,Rash Brian A.²,Rainey Fred A.²,Moe William M.¹

Affiliation:

1. Department of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, Louisiana 70803

2. Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803

Abstract

ABSTRACT Members of the haloalkane dechlorinating genus Dehalogenimonas are distantly related to “ Dehalococcoides ” but share high homology in some variable regions of their 16S rRNA gene sequences. In this study, primers and PCR protocols intended to uniquely target Dehalococcoides were reevaluated, and primers and PCR protocols intended to uniquely target Dehalogenimonas were developed and tested. Use of the genus-specific primers revealed the presence of both bacterial groups in groundwater at a Louisiana Superfund site.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01938-09

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