Strain Structure and Dynamics Revealed by Targeted Deep Sequencing of the Honey Bee Gut Microbiome

Author:

Bobay Louis-Marie1,Wissel Emily F.1,Raymann Kasie1ORCID

Affiliation:

1. Department of Biology, University of North Carolina at Greensboro, Greensboro, North Carolina, USA

Abstract

The factors driving fine-scale composition and dynamics of gut microbial communities are poorly understood. In this study, we used metagenomic amplicon deep sequencing to decipher the strain dynamics of two key members of the honey bee gut microbiome. Using this high-throughput and cost-effective approach, we were able to confirm results from previous large-scale whole-genome shotgun (WGS) metagenomic sequencing studies while also gaining additional insights into the community dynamics of two core members of the honey bee gut microbiome. Moreover, we were able to show that cryptic strains are not responsible for the observed variations in microbiome composition across bees.

Funder

National Science Foundation

HHS | NIH | National Institute of General Medical Sciences

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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