Affiliation:
1. Departamento de Microbiología, Universitat de València, Burjasot, Spain.
Abstract
The occurrence of drug resistance and its plasmid-mediated transferability was investigated in 140 environmental strains of Vibrio cholerae non-O1 and 6 strains of Vibrio cholerae, both O1 and non-O1, of clinical origin. Of the 146 strains tested, 93% were resistant to at least one drug and 74% were resistant to two or more antibiotics. The O1 strains were susceptible to all antibiotics used. A total of 26 of 28 selected resistant wild strains carried R plasmids that were transferable by intraspecific and intergeneric matings. The most common transmissible R factor determined resistance to ampicillin, amoxicillin, and sulfanilamide (30%), followed by resistance to ampicillin and amoxicillin (13%) and resistance to ampicillin, amoxicillin, phosphomycin, and sulfanilamide (9%). Comparison of the three methods of plasmid analysis showed that the method of Birnboim and Doly (Nucleic Acids Res. 7:1513-1523, 1979) without EDTA and lysozyme was optimal for isolation of both large and small plasmids in environmental V. cholerae strains. Most strains harbored more than one plasmid, and the molecular sizes ranged from 1.1 to 74.8 megadaltons. The plasmids of high molecular size (around 74 megadaltons) were responsible for the resistance pattern transferred and were maintained with high stability in the hosts.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference36 articles.
1. Afifi A. A. and S. P. Azen. 1972. Statistical analysis. A computer oriented approach. Academic Press Inc. (London) Ltd. London.
2. R-plasmid transfer in Salmonella isolated from wastewater and sewage-contaminated surface waters;Alcaide E.;AppI. Environ. Microbiol.,1984
3. Characterization of R-plasmids in environmental isolates of Salmonella: host range and stability;Aznar R.;Curr. Microbiol.,1988
4. Coincident plasmids and antimicrobial resistance in marine Atlantic ocean samples;Baya A. M.;Appl. Environ. Microbiol.,1986
5. Beringer J. E. and P. R. Hirsch. 1984. Genetic adaptation to the environment. The role of plasmids in microbial ecology p. 63-70. In M. J. Klug and C. A. Reddy (ed.) Current perspectives in microbial ecology. Proceedings of the 3rd International Symposium on Microbial Ecology Michigan State University. American Society for Microbiology. Washington D.C.
Cited by
22 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献