Multiplex PCRs for Identification of Necrotoxigenic Escherichia coli-Reference-Cited by-同舟云学术

Multiplex PCRs for Identification of Necrotoxigenic Escherichia coli

Published:2003-09 Issue:9 Volume:41 Page:4480-4482
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Van Bost Sigrid¹,Jacquemin Etienne¹,Oswald Eric²,Mainil Jacques¹

Affiliation:

1. University of Liège, Faculty of Veterinary Medicine, Laboratory of Bacteriology, Sart-Tilman B43a, B-4000 Liège, Belgium

2. Institut National de la Recherche Agronomique, Unité de Microbiologie Moléculaire, 31076 Toulouse Cedex, France

Abstract

ABSTRACT Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the adhesins. They were validated by single PCRs performed with reference E. coli strains and by multiplex PCRs with necrotoxigenic E. coli strains isolated from different animal species.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.9.4480-4482.2003

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