Affiliation:
1. Department of Biological Chemistry, Milton S. Hershey Medical Center, Pennsylvania State University 17033.
Abstract
The Escherichia coli K-12 genetic element, e14, contains a 216-base-pair region that is homologous to a portion of the host chromosome. This region serves as the integration site for the element. The 216-base-pair homology is interrupted by 28 mismatches distributed through the sequence. The actual integrative crossover occurs within the first 11 base pairs from one end of the region. To test factors which affect e14 site-specific recombination, we cloned the attachment sites of free e14 and the host chromosome into the same plasmid. The cloned attachment sites recombined intramolecularly in a process that required the presence of a chromosomal copy of e14 in the host cell as well as the induction of SOS. Recombination events that mimicked both integration and excision occurred under the same conditions and to roughly the same extent.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference17 articles.
1. Rapid chemical degradation sequencing;Bencini D. A.;Biotechniques,1984
2. A rapid alkaline extraction procedure for screening recombinant plasmid DNA;Birnboim H. C.;Nucleic Acids Res.,1979
3. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of EcoRI generated recombinant DNA molecules;Bolivar F.;Gene,1978
4. Excision and reintegration of the Escherichia coli K-12 chromosomal element e14;Brody H.;J. Bacteriol.,1985
5. Echols H. and G. Guarneros. 1983. Control of integration and excision p. 75-92. In R. W. Hendrix J. W. Roberts F. W. Stahl and R. A. Weisberg (ed.) Lambda II. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
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