Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae-Reference-Cited by-同舟云学术

Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae

Published:1991-11 Issue:11 Volume:29 Page:2517-2521
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Shirai H¹,Nishibuchi M¹,Ramamurthy T¹,Bhattacharya S K¹,Pal S C¹,Takeda Y¹

Affiliation:

1. Department of Microbiology, Faculty of Medicine, Kyoto University, Japan.

Abstract

We report a set of oligonucleotide primers and amplification conditions for the polymerase chain reaction to detect the ctx operon of Vibrio cholerae. The results of this assay on strains of V. cholerae and related organisms were identical with those obtained by the DNA colony hybridization test with the polynucleotide probe. The detection limit of this system was 1 pg of chromosomal DNA or broth culture containing three viable cells. The polymerase chain reaction method could directly detect the ctx operon sequences in rice-water stool samples from patients with cholera.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.29.11.2517-2521.1991

Reference21 articles.

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