Author:
An Gynheung,Hidaka Katsuhiko,Siminovitch Louis
Abstract
A recombinant plasmid containing the gene for bacterial β-galactosidase, situated close to the simian virus 40 early promoter, has been constructed. Transfection of CHO, L, and COS-1 cells with this plasmid led to the expression and appearance of the enzyme. Using this system, we have developed a series of promoter cloning vehicles capable of accepting promoter signals for animal genes.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
175 articles.
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