Identification of Secreted Antigen 3 from Babesia gibsoni-Reference-Cited by-同舟云学术

Identification of Secreted Antigen 3 from Babesia gibsoni

Published:2009-06 Issue:6 Volume:16 Page:944-948
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Jia Honglin¹²³,Terkawi M. Alaa¹²³,Aboge Gabriel Oluga¹²³,Goo Youn-Kyoung¹²³,Ma Liqing¹²³,Zhou Jinlin¹²³,Nishikawa Yoshifumi¹²³,Igarashi Ikuo¹²³,Fujisaki Kozo¹²³,Xuan Xuenan¹²³

Affiliation:

1. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan

2. Qinghai Academy of Animal and Veterinary Science, Weier Road, Xining, Qinghai 810003, China

3. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Ziyue Road, Minhang District, Shanghai 200241, China

Abstract

ABSTRACT A cDNA expression library of Babesia gibsoni was screened with the serum collected from a dog experimentally infected with B. gibsoni . A novel antigen sharing homology with secreted antigen 1 of B. gibsoni was isolated. The genomic analysis indicated that the BgSA3 gene exists as multicopies in the genome of B. gibsoni . The putative peptide encoded by the BgSA3 gene showed some characteristics of secreted proteins. The serum raised in mice immunized with the recombinant BgSA3 expressed in Escherichia coli could recognize a native parasite protein with a molecular mass of 70 kDa. Moreover, a sandwich enzyme-linked immunosorbent assay with anti-BgSA3 antibodies could detect the circulating BgSA3 in the blood plasma of dogs experimentally infected with B. gibsoni . The identification of BgSA3 provided a useful target for the development of a diagnostic test for detecting specific antibodies and circulating antigens.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00087-09

Reference25 articles.

1. Alifrangis, M., F. T. Ihristensen, C. S. Jorgensen, A. M. Ronn, J. E. Weng, M. Chen, I. C. Bygjerg, W. Sirawaraporn, Y. Palarasah, and C. Koch. 2004. Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from Plasmodium falciparum. Malar. J.12:3-16.

2. Benito, A., and D. Carmena. 2005. Double-antibody sandwich ELISA using biotinylated antibodies for the detection of Echinococcus granulosus coproantigens in dogs. Acta Trop.95:9-15.

3. Beniwal, R. P., A. K. Nichani, N. K. Rakha, R. D. Sharma, and S. Sarup. 1997. An immunization trial with in vitro produced Babesia bigemina exoantigens. Trop. Anim. Health Prod.29:124s-126s.

4. Boozer, A. L., and D. K. Macintire. 2003. Canine babesiosis. Vet. Clin. North Am. Small Anim. Pract.33:855-904.

5. Bork, S., M. Okamura, S. Boonchit, H. Hirata, N. Yokoyama, and I. Igarashi. 2004. Identification of Babesia bovisl-lactate dehydrogenase as a potential chemotherapeutical target against bovine babesiosis. Mol. Biochem. Parasitol.2:165-172.

Cited by 8 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Evaluation of recombinant BgSA3 protein based indirect-ELISA for sero-diagnosis and sero-surveillance of Babesia gibsoni in dogs;Veterinary Parasitology;2021-01

2. Babesia microti Protein BmSP44 Is a Novel Protective Antigen in a Mouse Model of Babesiosis;Frontiers in Immunology;2020-07-07

3. Genome-wide analysis of gene expression and protein secretion of Babesia canis during virulent infection identifies potential pathogenicity factors;Scientific Reports;2017-06-13

4. Identification of a thioredoxin reductase from Babesia microti during mammalian infection;Parasitology Research;2016-05-11

5. Development of recombinant BgP12 based enzyme linked immunosorbent assays for serodiagnosis of Babesia gibsoni infection in dogs;Veterinary Immunology and Immunopathology;2016-01