Affiliation:
1. Department of Microbiology, University of New Hampshire, Durham, New Hampshire 03824
Abstract
Beta-glucuronidase activity was investigated during a 48-h period in which virus replication and changes in cell morphology occurred. Infection of an established line of chimpanzee liver cells with either nononcogenic adenovirus 5 or highly oncogenic adenovirus 12 under one-step growth conditions produced differing patterns of enzyme activity. There was an increase in total activity and also enhanced leakage of beta-glucuronidase from cells infected with adenovirus 12. In contrast, the enzymatic pattern of cells infected with adenovirus 5 was similar to that of uninfected cells. Hydrocortisone prevented the abnormal release of beta-glucuronidase from adenovirus 12-infected cells. The compound had no effect on total enzyme activity or on virus replication and the development of cytopathology.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference20 articles.
1. Histochemical studies of lysosomes and Iysosomal enzymes
in
virus- infected cell cultures;Allison A. C.;J. Exp. Med.,1965
2. Differential release of cellular lactate dehydrogenase during replication of adenovirus types 5 and 12;Bardell D.;J. Gen. Virol.,1972
3. Inhibition of host protein synthesis in type 5 adenovirus-infected cells;Bello L. J.;J. Virol.,1967
4. Poliovirusinduced cellular injury;Blackman K. E.;J. Virol.,1969
5. Modifications enzymatiques du cytoplasme de cellules (lignee etablie T) infectees par l'adenovirus type 5. II. Enzymes des cellules infectees;Chardonnet Y.;Ann. Inst. Pasteur,1969
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献