Affiliation:
1. Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73069
Abstract
Cell wall and cytoplasmic fractions were isolated from mechanically disrupted yeast-phase cells of
Blastomyces dermatitidis
in an effort to obtain a reliable skin-test antigen. The biological activities of these fractions were compared with those of two blastomycin preparations. The cytoplasmic antigens exhibited a low index of specificity yet exceeded the specificities of the blastomycins. The skin test-active component(s) of the cytoplasmic material had a molecular weight between 10,000 and 30,000 and could easily be concentrated by ultrafiltration on a PM-10 membrane. Unlike the cytoplasmic antigens and blastomycins, an alkali-soluble, water-soluble cell wall antigen effectively distinguished guinea pigs that were sensitized to
B. dermatitidis
from those sensitized to
Histoplasma capsulatum.
The biological activity of the yeast-phase antigen could be quantitated, on a weight basis, after purification by ultrafiltration (PM-10 membrane).
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
48 articles.
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