Combined Real-Time PCR and Pyrosequencing Strategy for Objective, Sensitive, Specific, and High-Throughput Identification of Reduced Susceptibility to Penicillins in Neisseria meningitidis-Reference-Cited by-同舟云学术

Combined Real-Time PCR and Pyrosequencing Strategy for Objective, Sensitive, Specific, and High-Throughput Identification of Reduced Susceptibility to Penicillins in Neisseria meningitidis

Published:2008-02 Issue:2 Volume:52 Page:753-756
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Thulin Sara¹,Olcén Per¹,Fredlund Hans¹,Unemo Magnus¹

Affiliation:

1. National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Örebro University Hospital, Örebro, Sweden

Abstract

ABSTRACT A segment of penA in Neisseria meningitidis strains ( n = 127), including two nucleotide sites closely associated to reduced susceptibility to penicillins, was amplified and pyrosequenced. All results were in concordance with Sanger sequencing, and a high correlation between alterations in the two Pen i -specific sites and reduced susceptibility to penicillins was identified.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00914-07

Reference22 articles.

1. Nonculture Prediction of Neisseria meningitidis Susceptibility to Penicillin

2. Antignac, A., I. G. Boneca, J. C. Rousselle, A. Namane, J. P. Carlier, J. A. Vázquez, A. Fox, J. M. Alonso, and M. K. Taha. 2003. Correlation between alterations of the penicillin-binding protein 2 and modifications of the peptidoglycan structure in Neisseria meningitidis with reduced susceptibility to penicillin G. J. Biol. Chem.278:31529-31535.

3. Sequencing of Neisseria meningitidis penA Gene: the Key to Success in Defining Penicillin G Breakpoints

4. Gharizadeh, B., M. Akhras, M. Unemo, B. Wretlind, P. Nyrén, and N. Pourmand. 2005. Detection of gyrA mutations associated with ciprofloxacin resistance in Neisseria gonorrhoeae by rapid and reliable pre-programmed short DNA sequencing. Int. J. Antimicrob. Agents26:486-490.

5. Hopkins, K. L., C. Arnold, and E. J. Threlfall. 2007. Rapid detection of gyrA and parC mutations in quinolone-resistant Salmonella enterica using pyrosequencing technology. J. Microbiol. Methods68:163-171.

Cited by 4 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Techniques of Nucleic Acid-Based Diagnosis in the Management of Bacterial and Viral Infectious Diseases;Nucleic Acids as Molecular Diagnostics;2014-09-05

2. Real-time PCR as a diagnostic tool for bacterial diseases;Expert Review of Molecular Diagnostics;2012-09

3. Molecular diagnostics of infectious diseases;Current Opinion in Pediatrics;2009-02

4. Real-time PCR and subsequent pyrosequencing for screening ofpenAmosaic alleles and prediction of reduced susceptibility to expanded-spectrum cephalosporins inNeisseria gonorrhoeae;APMIS;2008-11