Improved Laboratory Safety by Decontamination of Unstained Sputum Smears for Acid-Fast Microscopy-Reference-Cited by-同舟云学术

Improved Laboratory Safety by Decontamination of Unstained Sputum Smears for Acid-Fast Microscopy

Published:2005-08 Issue:8 Volume:43 Page:4245-4248
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Giacomelli Luiz Roberto Bigão¹²,Helbel Cesar¹,Ogassawara Roger Leandro Nunes¹,Barreto Ângela Maria Werneck²,Martins Fátima Moreira²,Cardoso Celso Luíz¹,Leite Clarice Queico Fujimura³

Affiliation:

1. Departamento de Análises Clínicas, Universidade Estadual de Maringá, PR

2. Centro de Referência Professor Hélio Fraga, Rio de Janeiro, RJ

3. Faculdade de Ciências Farmacêuticas/UNESP, Araraquara, SP, Brazil

Abstract

ABSTRACT Tubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. The smears were decontaminated by the tube and slide techniques. Phenol at 5%, glutaraldehyde at 2%, and buffered formalin at 3.7% for 1 min (tube technique) or for 10 min (slide technique) were effective in decontaminating sputum smears and preserved cell morphology and quantitative acid-fast microscopy results.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.8.4245-4248.2005

Reference20 articles.

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