Evidence that Expression of the Vibrio vulnificus Hemolysin Gene Is Dependent on Cyclic AMP and Cyclic AMP Receptor Protein

Author:

Bang Young Bae1,Lee Shee Eun2,Rhee Joon Haeng23,Choi Sang Ho13

Affiliation:

1. Department of Food Science and Technology, Institute of Biotechnology, Chonnam National University, Kwang-Ju, 500-757,1 and

2. Department of Microbiology,2

3. Institute of Medical Sciences,3 Chonnam National University Medical School, Kwang-Ju, 500-190, South Korea

Abstract

ABSTRACT Glucose repressed hemolysin production in Vibrio vulnificus . Promoter activity of the hemolysin gene, vvh , assessed with a vvh-luxCDABE transcriptional fusion, required cyclic AMP (cAMP) and cAMP receptor protein (CRP) in Escherichia coli . Hemolysin production in V. vulnificus increased after the addition of cAMP and was undetectable in a putative crp mutant, suggesting that vvh is also regulated by cAMP-CRP in V. vulnificus .

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference23 articles.

1. Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein;Aiba H.;Nucleic Acids Res.,1982

2. Bachmann B. J. Derivations and genotypes of some mutant derivatives of Escherichia coli K-12 Escherichia coli and Salmonella: cellular and molecular biology 2nd ed. Neidhardt F. C. Curtiss R. III Ingraham J. L. Lin E. C. C. Low K. B. Magasanik B. Reznikoff W. S. Riley M. Schaechter M. Umbarger H. E. 1996 2460 2488 American Society for Microbiology Washington D.C.

3. Diseases of Humans (Other than Cholera) Caused by Vibrios

4. Cyclic AMP in prokaryotes;Boxford J.;Microbiol. Rev.,1992

5. Choy H. E. 1997. Unpublished data.

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