Freely moving recording:Chronic recoverable Neuropixels in mice v2

Author:

A Aery Jones EmilyORCID

Abstract

This protocol collection explains how to build a low-cost, lightweight system to implant 1 Neuropixels 1.0 probe or 2 Neuropixels 2.0 probes into mice, record during freely moving behavior, then recover the probes for future use. This protocol enables recording from mice after following the Assembly and Implant surgery protocols, or after any chronic electrode implantation. This protocol first explains how to build enclosures for spatial tasks, construct a frame to mount the enclosures and related hardware, and wire and code an Arduino to operate poke detectors, pinch valves to dispense reward, and floor-mounted doors. Second, it explains how these procedures can be used specifically to build an X-maze for mice. Third, it explains how to integrate recording by building a headfixation apparatus for easy headstage attachment and a pulley-operated counterweight to counteract the headstage weight and enable easier running. The X-maze is derived from the H maze, originally published in Seigle & Wilson, 2014 (https://elifesciences.org/articles/03061) with a detailed protocol published in Wirtshafter, Quan, & Wilson, 2021 (https://bio-protocol.org/e3947). See full collection for more details.

Publisher

ZappyLab, Inc.

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3