Vertical sleeve gastrectomy improves liver and hypothalamic functions in obese mice

Author:

Li Peixin123,Rao Zhijian234,Laing Brenton Thomas234,Bunner Wyatt234,Landry Taylor234,Prete Amber2,Yuan Yuan23,Zhang Zhong-Tao5,Huang Hu2346

Affiliation:

1. 1Department of Comprehensive Surgery, Medical and Health Center, Beijing Friendship Hospital, Capital Medical University, Beijing, People’s Republic of China

2. 2East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, North Carolina, USA

3. 3Human Performance Laboratory, College of Health and Human Performance, East Carolina University, Greenville, North Carolina, USA

4. 4Department of Kinesiology, East Carolina University, Greenville, North Carolina, USA

5. 5Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing Key Laboratory of Cancer Invasion and Metastasis Research & National Clinical Research Center for Digestive Diseases, Beijing, People’s Republic of China

6. 6Department of Physiology, East Carolina University, Greenville, North Carolina, USA

Abstract

Vertical sleeve gastrectomy (VSG) is an effective surgery to treat obesity and diabetes. However, the direct effect of VSG on metabolic functions is not fully understood. We aimed to investigate if alterations in hypothalamic neurons were linked with perturbations in liver metabolism after VSG in an energy intake-controlled obese mouse model. C57BL/6 and hrNPY-GFP reporter mice received HFD for 12 weeks and were then divided into three groups: Sham (ad lib), Sham (pair-fed) with VSG and VSG. Food intake was measured daily, and blood glucose levels were measured before and after the study. Energy expenditure and body composition were determined. Serum parameters, liver lipid and glycogen contents were measured and gene/protein expression were analyzed. Hypothalamic POMC, AgRP/NPY and tyrosine hydroxylase-expressing neurons were counted. The following results were obtained. VSG reduced body weight gain and adiposity induced by HFD, increased energy expenditure independent of energy intake. Fed and fasted blood glucose levels were reduced in the VSG group. While serum active GLP-1 level was increased, the active ghrelin and triglycerides levels were decreased along with improved insulin resistance in VSG group. Liver lipid accumulation, glycogen content and gluconeogenic gene expression were reduced in the VSG group. In the hypothalamus, TH-expressing neuron population was decreased, and the POMC-expressing neuron population was increased in the VSG group. In conclusion, our data suggest that VSG improves metabolic symptoms by increasing energy expenditure and lowering lipid and glycogen contents in the liver. These physiological alterations are possibly related to changes in hypothalamic neuron populations.

Publisher

Bioscientifica

Subject

Endocrinology,Endocrinology, Diabetes and Metabolism

Reference49 articles.

1. The effects of exercise on hypothalamic neurodegeneration of Alzheimer’s disease mouse model;PLoS ONE,2018

2. Regulation of glucose homeostasis by GLP-1;Progress in Molecular Biology and Translational Science,2014

3. Hypothalamic control of energy balance: insights into the role of synaptic plasticity;Trends in Neurosciences,2013

4. Effect of bariatric surgery on adipose tissue glucose metabolism in different depots in patients with or without type 2 diabetes;Diabetes Care,2016

5. Neuronal control of peripheral insulin sensitivity and glucose metabolism;Nature Communications,2017

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3