IDENTIFICATION DES CORTICOSTEROIDES SECRETES PAR LES SURRENALES EMBRYONNAIRES DE VEAU, LAPIN ET SOURIS CULTIVEES IN VITRO

Abstract

ABSTRACT Adrenals from calf, rabbit and mouse embryos were incubated with [1-14C] sodium acetate. The corticosteroids were extracted from the incubation media and identified by sequential thin-layer radiochromatography and derivative formation, either alone or combined with recrystallization to constant specific activity. It is concluded that adrenals from calf embryo synthesize in vitro cortisol, corticosterone and 11-dehydrocorticosterone; from rabbit embryo, cortisone, cortisol and aldosterone; and from mouse embryo, cortisol, corticosterone, 11-dehydrocorticosterone, 11-deoxycorticosterone and aldosterone.