INDICATORS OF WATER-SALT ВALANCE IN ANIMALS UNDER CONDITIONS OF DIFFERENT NUTRIENT SUPPLY

Abstract

The aim of this work was to study the indicators of water-salt balance in animals under conditions of different supply of sucrose and dietary protein. Blood levels of Na+, K+-ions were measured by method atomic emission spectroscopy, aldosterone level was determined by enzyme-linked immunosorbent assay method using Aldosterone ELISA kit. studies were performed on 3 groups of animals: I - intact animals (K); II – animals receiving high-sucrose diet (HS); III – animals receiving low-protein high-sucrose diet (LP/HS). It is established that significant disturbances of water-salt balance were observed in the group of animals kept on high-sucrose and low-protein/high-sucrose diet. In animals of the reduction of serum aldosterone content more than 2 times compared to control group values with concomitant hyponatremia and hyperkalemia was revealed, indicating the formation of ion imbalance. A simultaneous established increased osmolarity blood serum indicates the significant dehydration. Since no significant changes in water-salt metabolism are observed under the conditions of lowprotein diet consumption, it is concluded that excessive consumption of sucrose is a key factor in the violation of regulation of water-salt metabolism in animals. The obtained research results can be used to develop a strategy for correction of the disorders of water-salt balance under the conditions of nutritional imbalance.