Features of reparative myogenesis of the internal anal sphincter locally exposed to humic acids

Abstract

Aim to evaluate the effect of local exposure to humic substances on the repair of smooth muscle tissue of the internal anal sphincter. Material and methods. Laboratory male rats were subjects for experimental study. The tissues were studied using light-electron microscopy and immunocytochemistry. Results. The study revealed the features of myocyte survival in the wound and near-wound zones, the connective tissue scar formation rate. The phenomenon of the transformation of contractile myocytes into contractile-secretory ones was confirmed. The experiment has registered several features of the compensatory processes occurring with the use of humic acids as a local therapeutic agent. Specifically, the presence of a more pronounced edema of the interstitium, prolongation in time, in comparison with the control, filling the interstitium with connective tissue, as a result of which the architectonics of collagen fibers was adequate to the direction of myocyte contraction and relaxation. In addition, mitotically dividing cells were found among the muscle layer of the wound zone in the experimental group. Conclusion. The experimental data suggests that humic acids, being a natural factor, can contribute to the processes of intracellular and cellular regeneration, resulting in better restoration of the internal sphincter structure. Further development of humic substances along with the research on their action at the cellular and tissue levels should be encouraged.