Phytochemical Profiling and Evaluation for Anti-oxidant, Thrombolytic, and Antimicrobial Activities of Moringa oleifera Lam Leaves Extracts

Abstract

The present study was undertaken for phytochemical screening as well as evaluating in vitro antioxidant, thrombolytic, and antimicrobial properties of crude methanolic extracts of Moringa oleifera Lam leaf and its diversified extractives. The antioxidant potential was investigated by ascertaining the capability of the samples to DPPH scavenging with phenol compositions estimated. Besides, thrombolytic and antibacterial properties were assessed following clot lysis and disc diffusion methods, respectively. All fractions were found to contain flavonoids, reducing sugars, tannins, gums, saponins, and quinines, while ME, PESF and CTSF comprise glycosides, steroids, and terpenoids. In the antioxidant activity assay, CTSF possessed the uppermost phenolic content (34.38 mg of GAE/gm) and the scavengers of DPPH free radicals potential with IC50 value of 2.96 µg/mL associated to the standard drug ascorbic acid (2.48µg/mL). AQSF displayed the highest percentage of clot lysis (25.00%), compared to the conventional drug streptokinase (63.74%). The methanol extract, PESF and CSF of M. oleifera displayed antimicrobial activity against all tested microorganisms. Therefore, the outcomes of the existing study were accompanied by the validation of the antioxidant, thrombolytic, and antimicrobial properties of M. oleifera leaves, which justified the plant's use in traditional medicine.