Quantification of Marker Compound by HPTLC-densitometry Method and Assessment of Anti-inflammatory Activity of Root-bark Extract of Berberis aristata with Molecular Docking by COX-1 and COX-2

Abstract

Berberis aristata commonly known as Daruhaldi, which is traditionally used in the various treatment such as inflammation, irritation, wound and other skin aliments. During the tribal time period, Indian people were use the crused from of root-bark directly on infected parts of skin. Most of the people used the matured root bark in crused form and directly apply a thick paste on the inflammated surface. In this investigation, quantify the important marker compound (Berberine and Tetrahydropalmatine) by the HPTLC-densitometry method and further evaluate the anti-inflammatory activity of EEBA and also done the molecular docking, which was done by using Auto-Dock software. Croton oil induced ear oedema and carrageenan induced paw oedema model was used to evaluate the anti-inflammatory efficacy. Auto-Dock software was used to determine the molecular mechanism. The finding of the HPTLC-densitometry validated method revealed that the RSD for interday (0.113-0.343 and 0.116-0.342) and intraday (0.101-0.304 and 0.105-0.296) are as per ICH guidelines. The pharmacological evaluation of anti-inflammatory activity revealed a significant reduction of oedema when using 4% formulation. On behlf of molecular docking berberine and tetrahydropalmatine inhibit the of COX-1 and COX 2 levels. It was concluded that the application of the extract in animals to be used as an anti-inflammatory agent for topical formulations and the phytoconstituent have potentcy to inhibit the effect of COX-1 & 2.