HPLC Purification of Bio-Actives of Cleome Viscosa and their Anticancer Activity Against Breast Cancer Cell Lines

Abstract

Cleome viscosa, often known as Jakhya, is a common weed that may be found in farms, gardens, and a variety of other areas. Because of its significant anti-disease effect, the plant and its parts have been employed in traditional medicine. Aim: Current study aim to purify the bioactive having potent antioxidant activity and anticancer activity against BT-474 cell lines. Methods: Cleome viscosa leaf methanolic extracts were purified through preparative HPLC and the isolated compounds were checked against MCF10A (normal cell line) and BT-474 (breast cancer cell line). Results: HPLC isolated components from methanolic leaves extract included atropine, nevirapine, gallic acid, caffeic acid, vanillic acid, and kaempferitrin. Compounds were more efficient in terms of DPPH. Kaempferitrin inhibited DPPH by 94%. Atropine, vanillic acid, and kaempferitrin were shown to be cytotoxic to BT474 cell lines, with IC50 values of 18.87, 1.316, and 46.42 µg/ml, respectively. Incubating with their IC50 concentrations caused no morphological harm to the cell. Only the number of cells decreased significantly as incubation time increased. Conclusion: Cleome viscosa purified compounds showed good antioxidant and anticancer activity. The compounds were found to be effective against BT474 breast cancer cell line.