Baseline Kinetics of Clot Waveform Analysis of Clotting Screening Tests on Sysmex CS2500 Automated Coagulometry

Abstract

Clot waveforms generated by different automated coagulometry are of immense utility in predicting the risk of bleeding or thrombosis. They are part of global hemostasis testing. Different automated analyzers use different technology for clotting screening tests. The clotting screening results depends on the instrument and the reagents used. Hence clot waveform analysis may have different kinetics based on instrument or reagents used. A baseline characteristic can help in clinical interpretation.Total 47 voluntary blood donors (23 males and 24 females) between 20-50 years age are included in the study. We have analyzed the kinetics of clot waveform of Prothrombin time (PT) using Dade Innovin, activated partial thromboplastin time (aPTT) using Actin FS & Actin FSL and thrombin time (TT) on Sysmex CS2500 automated coagulometry. The baseline characteristics were analyzed and the reference intervals were determined as follows: PT using Dade Innovin reagent: 9.8 – 12.0s, its 1st derivative or maximum velocity (min1): 1.00-2.42 %/s, 2nd derivative or maximum acceleration (min2): 0.14-0.38%/s2, maximum deceleration (max2): 0.18-0.42 %/s2; aPTT using actin FSL: 26.2-34.5s, min1 2.73-6.17%/s, min2 0.41-0.95%/s2, max2 0.30-0.77 %/s2 ; aPTT using actin FS reagent: 21.2-30.5s, min1 2.73-6.33 %/s, min2 0.37-1.07 %/s2, max2 0.32-0.94 %/s2 ; TT: 16.8-19.6 s, min1 0.57-1.23 %/s, min2 0.08-0.20 %/s2, max2 0.03-0.11 %/s2 respectively. The clotting time shows an inverse moderate to weak relationship with maximum velocity, maximum acceleration and maximum deceleration. Analyzing the normal kinetics of clot waveform helps in understanding the clotting process and clinical interpretation.