Analysis of serum homocysteine in the laboratory practice - comparison of the direct chemiluminescence immunoassay and high performance liquid chromatography coupled with fluorescent detection

Author:

Paprotny Łukasz1,Wianowska Dorota2,Izdebska Magdalena3,Celejewska Agnieszka1,Szewczak Dorota1,Solski Janusz3

Affiliation:

1. Research and Development Center, Alab Laboratories, Lublin, Poland

2. Department of Chromatographic Methods, Faculty of Chemistry, Maria Curie-Skłodowska University, Lublin, Poland

3. Department of Laboratory Diagnostics, Medical University of Lublin, Lublin, Poland

Abstract

Introduction: Effective diagnosis of cardiovascular diseases requires the right tools to be used enabling selective and sensitive analysis of their biomarkers. One of them is homocysteine (Hcy), nowadays determined by immunoassays and chromatographic methods. This study aims to compare the results obtained by direct chemiluminescence immunoassay (CLIA) and high performance liquid chromatography with fluorescent detection (HPLC-FD) using commercial kits. Materials and methods: Homocysteine concentration was determined in serum samples obtained from 101 individuals, using Atellica IM HCY (Siemens Healthineers, Erlangen, Germany) and HCY in plasma/serum – HPLC-FD (Chromsystems Instruments & Chemicals GmbH, Gräfelfing, Germany) tests validated for routine analysis. The latter was applied as a reference method. The comparability and agreement between the tested methods were evaluated using the Passing-Bablok (PB) regression analysis and the Bland-Altman (BA) method of the differences analysis. Results: Studies showed that CLIA gives higher Hcy concentrations (15.7 ± 4.14 μmol/L). Passing-Bablok regression analysis of the results obtained with CLIA (y) compared with HPLC-FD (x) yielded an intercept of 0.22 (95%CI: - 2.16 to 2.46) and slope of 1.58 (95%CI: 1.33 to 1.87). Bland-Altman analysis demonstrated a systematic positive bias for CLIA of 5.85 ± 2.77 μmol/L. Conclusions: Methods disagreement precludes their interchangeability. Lower Hcy values by HPLC-FD result from its greater selectivity. High performance liquid chromatography with fluorescent detection should be considered as preferential method for analysing Hcy in blood serum as well as the recommended reference method for routine clinical analysis. This fact, however, imposes the need to establish new reference ranges.

Publisher

Croatian Society for Medical Biochemistry and Laboratory Medicine

Subject

Biochemistry, medical,Clinical Biochemistry

Reference27 articles.

1. Cardiovascular diseases, health topics. World Health Organization. Available at: https://www.who.int/health-topics/cardiovascular-diseases/. Accessed December 22th 2019.

2. Homocysteine and homocysteine-related compounds: an overview of the roles in the pathology of the cardiovascular and nervous systems.;Djuric;Can J Physiol Pharmacol,2018

3. Role of homocysteine in the development of cardiovascular disease.;Ganguly;Nutr J,2015

4. Homocysteine and hyperhomocysteinaemia.;Zaric;Curr Med Chem,2019

5. Role of homocysteine in the development of cardiovascular disease.;Ganguly,2015

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