AbstractThis chapter reviews various horse handling methods, including imprint training and positive methods of training, horse transport and issues related to horse slaughter. The review of imprint training studies indicates that there have been mixed positive and negative results, and that the process may interfere with foal bonding with the mare. Brushing and stroking the mare will facilitate approaching and handling the foal. Positive methods of training, such as clicker training, are very helpful for training horses to enter a trailer. Horses can become stressed when they travel alone, and the position facing rear to the direction of travel may be less stressful. Stalls in horse trailers should be designed so that horses can see each other. During transport, horses will drink less water and there should be stops every 16 to 24 h for feed and water. Since the US horse slaughter plants were closed, horse rescue groups are overwhelmed. In Europe, transport laws are not enforced, and one-third of the slaughter horses that arrive in Italy are not fit for transport. Cortisol levels are higher after transport than after stunning at slaughter.