Net flux of nutrients across splanchnic tissues in wethers consuming grasses of different sources and physical forms ad libitum

Abstract

Crossbred sheep (n 16,8·5 months of age and 33 (SE 0·9) kg) were used in a 21 d experiment (2x2 factorial) to determine effects on net flux of nutrients across the portal-drained viscera (PDV) and liver of ad libitum consumption of bermudagrass (Cynodon dactylon; B) v. ryegrass (Lolium multiflorum)-wheat (Triticum aestivum; RW) hay, coarsely chopped (CC) or finely ground and pelleted (GP). Crude protein concentrations were 86, 81, 113 and 119g/kg and neutral-detergent fibre concentrations were 710, 688, 654 and 672 g/kg (dry matter basis) for B-CC, B-GP, RW-CC and RW-GP respectively. Digestible energy intake (6.0,9.6·, 10·2 and 13·8 W/d) Mered (P < 0·01) with grass source and form, and digestible N intake values were 4·4, 7·0, 8·4 and 14.1 (SEM 0·82) g/d for B-CC, B-GP, RW-CC and RW-GP diets respectively. Consumption of O2 by the PDV (118,165,144 and 155mmol/h) and splanchnic bed (196,273,247 and 266 mmollh for B-CC, B-GP, RW-CC and RW-GP respectively) was greater (P=O·O7) for GP than for CC. The ratio splanchnic heat energy production: digestible energy intake was greater (P=0·06) for B than for RW (0·374,0·300,0·278 and 0·219 for B.CC, B-GP, RW-CC and RW-GP respectively). α-Amino-N release by the PDV (P< 0·01; 11·6, 12·8, 23·0 and 18·7 mmoyh) and uptake by the liver (P=0·07; 15·2, 6·1, 17·0 and 19·3 mmol/h for B-CC, B-GP, RW-CC and RW-GP respectively) were greater for RW than for B. Release of NH3-N by the PDV was greater (P=O·02) for CC than for GP (12·5, 6·2, 15·7 and 8·9 mmol/h), and hepatic urea-N release differed between grass sources (P=O·O3) and physical forms (P=0·07; 22·6, 12·7, 31·4 and mmol/h for B-CC, B-GP, RW-CC and RW-GP respectively). In conclusion, decreases in forage particle size elicited by grinding and pelleting did not affect the difference between grass sources in splanchnic tissue heat energy production relative to digestible energy intake.