Nitrogen metabolism and urinary excretion of purines in goat kids

Abstract

1. In Expt 1 three male goat kids of the Swedish Landrace breed were bottle-fed on isoenergetic liquid diets composed of goat's milk alone or substituted with 200, 400, 600 and 800 ml of a nitrogen-free liquid diet/1. The goat kids were 3 weeks old at the start of the experiment and weighed on average 5.3 (sd 0.22) kg. The experiment lasted for 45 d divided into nine 5 d periods. The goat kids were kept individually in metabolism cages, and faeces and urine were collected daily.2. In Expt 1 there was a significant (P< 0.001) relation between N intake and N retention, with an estimated (extrapolation) basal N excretion of 211 mg N/kg metabolic live weight (W0.75). While the creatinine excretion remained fairly constant (19.9 mg/W0.75), there was a tendency for both the allantoin and uric acid excretions to change with N intakes. Hypoxanthine showed no consistent excretion pattern, and xanthine could not be detected.3. In Expt 2 three male goat kids of the Swedish Landrace breed were used to study the effect of level of intake on N and purine metabolism. The first experimental period (period 1) started at 2 weeks of age (5.5 kg live weight) and the second experimental period (period 2) at 9 weeks of age (9.8 kg live weight). The goat kids were bottle-fed on goat milk at intended intakes of 400, 600, 800 and 1000 kJ gross energy/kg W0.75. Each treatment was given for 7 d with adaptation for 3 d and collections for 4 d.4. In Expt 2 there were significant (P< 0.001) increases in both live weight and N retention when level of intake was increased. With increasing N intakes both total N and urea-N excretions increased significantly (P< 0.05). The proportions of urea-N and ammonia-N were fairly constant within periods, and were on average respectively 0.81 and 0.059 in period I and 0.84 and 0.068 in period 2. There was no significant effect of treatment on the urinary excretion of allantoin, hypoxanthine, xanthine and creatinine. In period 2 uric acid excretion was significantly (P< 0.01) affected by the treatments.5. From the presented findings it is concluded that the endogenous urinary excretion of purine derivatives in a young growing ruminant was only marginally affected by large variations in protein supply, and also by the level of intake of milk.