Inhibition of Clostridium botulinum Okra B by N-Acyl Amino Acid Ester (Nα-Cocoil Arginine Ethylester•DL-Pyrrolidone Carbonate)

Abstract

An amino acid ester, Nα-cocoil-L-arginine ethylester·DL-pyrrolidone carbonate (CAE), was inhibitory to growth and toxin production of Clostridium botulinum okra in peptone-yeast extract-glucose (PYG) medium, pH 7.0, at 30°C. Addition of 10 mg of CAE/L to PYG medium delayed toxin production and 25 mg of CAE/L inhibited growth and toxin production, whereas 5 mg of CAE/L had no effect on both growth and toxin production.