Prevalence, Serovars, and Antimicrobial Resistance of Salmonella in Cecal Samples of Chickens Slaughtered in Pluck Shops in Trinidad

Abstract

ABSTRACT The aim of the study was to determine the prevalence and zonal distribution of Salmonella serotypes in poultry and to determine the antimicrobial resistance profile of Salmonella isolates. A total of 1,503 cecal samples of poultry were randomly collected from 33 pluck shops across Trinidad. Isolation and identification of Salmonella followed standard methods, and the disk diffusion method was used to determine resistance of isolates to 14 antimicrobial agents. Ninety-one (6.1%) of the 1,503 samples collected from four zones were positive for Salmonella. The frequency of isolation of Salmonella from chicken ceca (6.5%) was higher than that detected in duck ceca (5.1%), but the difference was not statistically significant (P > 0.05). Ten serotypes were detected, with Salmonella Molade, Salmonella enterica subsp. enterica I, and Salmonella Typhimurium the most prevalent at 56.0, 11.0, and 8.8%, respectively. The highest frequency of isolation of Salmonella was recorded in the northeast zone (59.3%). All 91 isolates exhibited resistance to at least 1 of the 14 antimicrobial agents. The highest frequency of resistance was exhibited to ampicillin (51.0%), kanamycin (49.5%), and streptomycin (37.4%). A total of 22 resistance patterns were exhibited by the 91 isolates of Salmonella, and 13 isolates (14.3%) exhibited multiple drug resistance. The results emphasize the need to implement hygienic practices to reduce the levels of contamination at poultry pluck shops and the need for prudent use of antimicrobial agents in the poultry production system in Trinidad.